Enhanced photo-fermentative H2 production using Rhodobacter sphaeroides by ethanol addition and analysis of soluble microbial products

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Enhanced photo-fermentative H2 production using Rhodobacter sphaeroides by ethanol addition and analysis of soluble microbial products

BACKGROUND Biological fermentation routes can provide an environmentally friendly way of producing H2 since they use renewable biomass as feedstock and proceed under ambient temperature and pressure. In particular, photo-fermentation has superior properties in terms of achieving high H2 yield through complete degradation of substrates. However, long-term H2 production data with stable performan...

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Microbial Biohydrogen Production by Rhodobacter sphaeroides O.U.001 in Photobioreactor

Pure strains of Purple Non-Sulfur (PNS) bacteria: Rhodobacter sphaeroides strain O.U 001 was studied for its efficiency to produce biohydrogen using the photofermentative pathways. An annular photobioreactor was being designed aiming the uniform light distribution. In prototype annular photobioreactor, at an initial pH of 6.8±0.2, temperature 32 ± 2 ◦C, inoculum volume 10% (v/v), inoculum age o...

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Effect of carbon and nitrogen sources on photo-fermentative H2 production associated with nitrogenase, uptake hydrogenase activity, and PHB accumulation in Rhodobacter sphaeroides KD131.

During photo-fermentative H(2) production, the effects of carbon and nitrogen sources on nitrogenase and hydrogenase activity, poly-β-hydroxybutyrate accumulation were investigated. In succinate/ammonium sulfate medium, H(2) was not detected for the first 6h because high ammonium concentration considerably reduced the nitrogenase activity to below 5 nmol/g-dcw/h. After 24h, 99% of the ammonium ...

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Complexes of Rhodobacter sphaeroides and Rhodobacter capsulatus

Photosynthetic bacteria offer excellent experimental opportunities to explore both the structure and function of the ubiquinol-cytochrome c oxidoreductase (bcl complex). In both Rhodobacter sphaeroides and Rhodobaeter capsulatus, the bCl complex functions in both the aerobic respiratory chain and as an essential component of the photosynthetic electron transport chain. Because the bc~ complex i...

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Pure-culture growth of fermentative bacteria, facilitated by H2 removal: bioenergetics and H2 production.

We used an H2-purging culture vessel to replace an H2-consuming syntrophic partner, allowing the growth of pure cultures of Syntrophothermus lipocalidus on butyrate and Aminobacterium colombiense on alanine. By decoupling the syntrophic association, it was possible to manipulate and monitor the single organism's growth environment and determine the change in Gibbs free energy yield (DeltaG) in ...

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ژورنال

عنوان ژورنال: Biotechnology for Biofuels

سال: 2014

ISSN: 1754-6834

DOI: 10.1186/1754-6834-7-79